Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 87-91.doi: 10.3969/j.issn.1673-8225.2011. 01.019

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Effects of siRNA target gene B7H1 on adhesion and migration of human placenta mesenchymal stem cells

Zhang Si-ying1, Wang Guo-yan1, Du Hai-bo2, Luan Xi-ying1   

  1. 1Department of Immunology, Binzhou Medical University, Yantai  264003, Shandong Province, China
    2Department of Gynaecology and Obstetrics, Laishan First People’s Hospital, Yantai  264000, Shandong Province, China
  • Received:2010-07-29 Revised:2010-09-07 Online:2011-01-01 Published:2011-01-01
  • Contact: Luan Xi-ying, Doctor, Professor, Department of Immunology, Binzhou Medical University, Yantai 264003, Shandong Province, China xiyingluan@yahoo.com.cn
  • About author:Zhang Si-ying★, Studying for master’s degree, Department of Immunology, Binzhou Medical University, Yantai 264003, Shandong Province, China nightpalm@163.com
  • Supported by:

    the Natural Science Foundation of Shandong Province, No. Y2006C02; the Medical Science and Technology Development Program of Shandong Province, No.2007HZ039; the Science Research Foundation of Binzhou Medical University, No. Y2007KYQD09

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Abstract:

BACKGROUND: Human placenta mesenchymal stem cells (hPMSCs) express costimulatory molecules B7H1 which plays an important role in its immunosuppressive capabilities. However, the effect of B7H1 on the biological behavior of hPMSCs has not been reported.
OBJECTIVE: To investigate the effect of B7H1 blockade on adhesion and migration of hPMSCs.
METHODS: hPMSCs were isolated from mature placenta by the method of digestion. Then in vitro hPMSCs were cultured, expanded and were used in test after third passage. Flow cytometry and RT-PCR analysis were used to investigate the expression of B7H1 on hPMSCs. Specific siRNAs of B7H1 were transfected into hPMSCs via cathodolyte liposome transfection method. RT-PCR was used to detect the changes of B7H1 gene expression. Then the adhesion of hPMSCs was determined by cell count, and the migration of hPMSCs was evaluated using Transwell method.
RESULTS AND CONCLUSION: siRNA could effectively block the expression of B7H1 which highly expressed on hPMSCs. After seeded for half an hour, the cell adhesion rate had no significantly difference in the blockade group and the control group. For 1 hour or 3 hours, however, the adhesion rate of hPMSCs was significantly higher in the blockade group than in the control group (P < 0.05). Transwell assay indicated that the migration numbers of hPMSCs in the blockade group were significantly less compared with the control group under the culture conditions of SDF-1α, DMEM-LG complete medium or hPMSCs culture supernatant (P < 0.01). The results showed that the adhesion ability of hPMSCs was enhanced while the ability of migration was inhibited after B7H1 blockade and suggested that B7H1 plays an important role in the adhesion and migration of hPMSCs.

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